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  3. Agrisera專業經典RUBISCO/Carbon metabolism抗體

    西美杰代理的瑞典Agrisera成立于1980年,作為全球知名植物抗體研發生產商,致力于研發高品質植物單克隆及多克隆抗體。Agrisera公司擁有1000多種用于植物和藻類研究的一抗,主要集中于逆境、生理、病理等相關蛋白抗體。Agrisera一直為廣大科研工作者提供熱門研究領域的抗體,例如:Loadingcontrol抗體、Mitochondrial抗體、Photosynthesis抗體、Environmentalstress-related抗體、Rubisco抗體等。本篇文章小編將著重推薦Agrisera經典臻品Rubisco/Carbonmetabolism抗體。

     


     

     

     


    1、Rubisco抗體

    Rubisco(核酮糖-1,5-二磷酸羧化酶)是光合作用中決定碳同化速率的關鍵酶,同時也是植物光呼吸的關鍵酶。從紫色細菌到植物,它的同源性已經得到了證實,并且由兩個蛋白質亞基組成,每個亞基有8個拷貝。在植物和綠藻中,大亞基(~55KDa)由葉綠體rbcl基因編碼,小亞基(15kDa)由核rbcs基因家族編碼。Agrisera的Rubisco相關抗體已驗證可與多種植物和藻類有反應性,如擬南芥、芹菜、青蒿等。

    部分產品信息:

    貨號

    品名

    實驗類型

    AS03 037

    RbcL | Rubisco large subunit, form I (rabbit)

    IL、IF、IG、TP、WB

    AS03 037A

    RbcL | Rubisco large subunit, form I (affinity purified)

    WB

    AS03 037-HRP

    RbcL | Rubisco large subunit, form I, HRP-conjugated (40 μg)

    ELISA、WB

    AS15 2955

    RbcL II | Rubisco large subunit, form II

    WB

    AS07 259

    RbcS | Rubisco small subunit (SSU)

    WB

    AS07 259A

    RbcS | Rubisco small subunit (SSU) (affinity purified)

    ELISA、WB


    部分產品應用示例


    AS03037


    AS152955

     
    部分產品參考文獻:

    [1]Wang et al. (2020). Effects and Mechanisms of Foliar Application of Silicon and Selenium Composite Sols on Diminishing Cadmium and Lead Translocation and Affiliated Physiological and Biochemical Responses in Hybrid Rice (Oryza Sativa L.) Exposed to Cadmium and Lead. Chemosphere. 2020 Jul;251:126347. doi: 10.1016/j.chemosphere.2020.126347.


    [2]Khajuria et al. (2020). Photochemical Efficiency Is Negatively Correlated With the Δ 9- Tetrahydrocannabinol Content in Cannabis Sativa L. Plant PhysiolBiochem. 2020 Apr 8;151:589-600. doi: 10.1016/j.plaphy.2020.04.003.


    [3]Kurmayer et al. (2020). Chemically labeled toxins or bioactive peptides show a heterogeneous intracellular distribution and low spatial overlap with autofluorescence in bloom-forming cyanobacteria. Sci Rep. 2020 Feb 17;10(1):2781. doi: 10.1038/s41598-020-59381-w. (Immunofluorescence)


    [4]Zhang et al. (2020). Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency-induced senescence in soybean. Plant Cell Environ. 2020 Feb 3. doi: 10.1111/pce.13736.


    [5]Zav?el et al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.

     

    2、Carbonic anhydrase抗體

    碳酸酐酶(Carbonicanhydrase,CA)是一種含鋅金屬酶。催化CO2和HCO3的可逆相互轉化,它在許多涉及脫羧或羧化反應的生理功能中發揮重要作用,包括光合作用和呼吸作用。Agrisera的Carbonic anhydrase相關抗體已驗證可與衣藻有反應性。

    部分產品信息

    貨號

    品名

    實驗類型

    AS05 073

    CAH3 | Carbonic anhydrase

    IF、WB

    AS19 4322

    CAH6 | Carbonic anhydrase 6

    WB


    部分產品應用示例:


    AS05073

    部分產品參考文獻:

    [1]Terentyev (2020).The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..


    [2]Terentyev et al. (2019). Carbonic anhydrase CAH3 supports the activity of photosystem II under increased pH.BiochimBiophys Acta Bioenerg. 2019 Jul 1;1860(7):582-590. doi: 10.1016/j.bbabio.2019.06.003.

     

    3、Phosphoenolpyruvate carboxylase抗體

    磷酸烯醇丙酮酸羧化酶(Phosphoenolpyruvate carboxylase,PEPC),EC=4.1.1.31,屬于羧化酶家族。在PEPC催化下,CO2與磷酸烯醇丙酮酸(PEP)結合生成草酰乙酸。磷酸烯醇丙酮酸羧化酶(PEPC)是碳同化途徑中最重要的酶之一,無論C3還是C4植物中都存在。

    部分產品信息:

    貨號

    品名

    實驗類型

    AS09 458

    PEPC | Phosphoenolpyruvate carboxylase

    IL、WB

    AS09 458-HRP

    PEPC | phosphoenolpyruvate carboxylase, HRP-conjugated (40 μg)

    WB

    AS09 458-ALP

    PEPC | phosphoenolpyruvate carboxylase, ALP-conjugated (40 μg)

    WB


    部分產品應用示例:


    AS09458

    部分產品參考文獻:

    [1]Durallet al. (2020). Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels. 2020 Jan 28;13:16. doi: 10.1186/s13068-020-1653-y.


    [2]Wanget al. (2019). PUB25 and PUB26 Promote Plant Freezing Tolerance by Degrading the Cold Signaling Negative Regulator MYB15.


    [3]Hui et al. (2018). TALE-carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice. Mol Plant Pathol. 2018 Nov 30. doi: 10.1111/mpp.12772.

     

    4、Phosphoribulokinase抗體

    磷酸核酮糖激酶(Phosphoribulokinase,PRK)是卡爾文循環的關鍵酶,消耗光反應過程中產生的ATP。Agrisera的Phosphoribulokinase抗體已驗證可用于擬南芥、玉米、水稻等植物。

    部分產品信息:

    貨號

    品名

    實驗類型

    AS07 257

    PRKribulose-5-P-kinase | Phosphoribulokinase

    WB

    AS07 257-ALP

    PRK ribulose-5-P-kinase | phosphoribulokinase, ALP-conjugated (40 μg)

    WB

    AS07 257-HRP

    PRK ribulose-5-P-kinase | Phosphoribulokinase, HRP-conjugated (40 μg)

    WB


    部分產品應用示例:


    AS07257

    部分產品參考文獻:

    [1]Fukayamaet al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9.


    [2]Pérez-Ruiz et al. (2017). NTRC-dependent redox balance of 2-Cys peroxiredoxins is needed for optimal function of the photosynthetic apparatus. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12069-12074. doi: 10.1073/pnas.1706003114.

     

    除上述介紹外,Agrisera還為廣大植物科研客戶提供前沿研究所需的TRX、Lci5、SBP等高品質已驗證抗體,更多產品信息獲取可登陸西美杰官網:www.xmjsci.com;或撥打全國客服電話400-050-4006咨詢。

    西美杰作為Agrisera中國區代理,為廣大客戶提供優質的已驗證抗體和售前售后服務支持。同時西美杰整合全球高品質植物學品牌:PhytoTech和PlantCellTechnology;免疫學品牌:KPL、Lifespan、Cali-Bio,為科研客戶提供一站式服務,解決科研客戶選擇困難、質量擔憂等問題。

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